Nyocha nke Nchekwa na arụmọrụ nke ọgwụgwọ Neurotrophic factor na Ischemic Stroke

Ọrịa strok nke ụbụrụ, nke a makwaara dị ka "stroke" ma ọ bụ "ihe mberede cerebrovascular" (CVA), bụ nnukwu ọrịa cerebrovascular nke a na-eji na mberede mgbawa nke arịa ọbara n'ụbụrụ ma ọ bụ igbochi ọbara na-eruba n'ụbụrụ, na-ebute mmebi nke ụbụrụ ụbụrụ. Ọ na-agụnye ma ischemic na hemorrhagic ọrịa strok. Ọrịa strok bụ ihe nke abụọ na-ebute ọnwụ n'ụwa niile, yana ọnụ ọgụgụ ndị okenye na-anwụ na nkwarụ dị elu.

Ischemic Stroke (IS) na-ezo aka na mpaghara ischemic necrosis ma ọ bụ ime ka anụ ahụ dị nro na-akpata site na ọrịa mgbasa cerebrovascular, ischemia, na hypoxia. N'ihi ọnụ ọgụgụ ya dị elu, njupụta ya, ọnụ ọgụgụ ndị na-anwụ anwụ na nkwarụ, ọ ghọwo otu n'ime isi ihe na-ebute ibu ọrịa zuru ụwa ọnụ. Dị ka ọnụ ọgụgụ si kwuo, ihe dị ka nde mmadụ 15 n'ụwa nile na-arịa ọrịa strok kwa afọ, yana nde mmadụ 5 na-anwụ na nde mmadụ 5 na-enwe nkwarụ ogologo oge.

Usoro ọgwụgwọ Stem Cell maka ọrịa strok ischemic

Mesenchymal Stem Cells (MSCs) bụ ụdị mkpụrụ ndụ stem nke nwere ike kewapụ iche na anụ ahụ dị iche iche, gụnyere ụmị ọkpụkpụ, anụ ahụ adipose, na ọbara eriri nwa. N'ihi ihe ha nwere ike ime, ha nwere ike ịwapụta n'ime osteoblasts, chondrocytes, adipocytes, na ọbụna neurons. Ugbu a, ntụgharị MSC apụtala dị ka usoro ọgwụgwọ ọhụụ maka ọrịa ischemic, gụnyere ọrịa strok, ọrịa akwara obi, na ọrịa akwara dị n'akụkụ. Ọtụtụ ọmụmụ egosila na mmetụta neuroprotective dị mma nke MSC bụ isi ihe na-agwọ ọrịa strok ischemic.

Akụkọ nyocha nke ugbu a na-egosi na MSC na-akwalite nrụzi mmebi nke anụ ahụ neural, mweghachi nke ọrụ akwara, mbelata ogo infarction ụbụrụ, na nkwalite neurogenesis na angiogenesis site na mmetụta paracrine, mmetụta immunomodulatory, na mmetụta mgbochi apoptotic, si otú a na-arụ ọrụ ọgwụgwọ. na ọrịa strok ischemic.

Mmetụta paracrine

Ihe paracrine nke MSC, gụnyere exosomes na extracellular vesicles, bụ isi ihe na-enye aka na mmetụta ọgwụgwọ ha. Na mpaghara mmerụ ahụ nke ụbụrụ ụbụrụ, MSC nwere ike ime ka nkwupụta nke ihe dị iche iche na-eto eto, gụnyere ụbụrụ ụbụrụ neurotrophic factor (BDNF), vascular endothelial growth factor (VEGF), stromal cell-derived factor 1 (SDF-1), isi fibroblast. ihe na-eto eto (bFGF), glial cell-derived neurotrophic factor, na ihe na-eto eto hepatocyte, n'etiti ndị ọzọ. Ihe ndị a na-enyere aka belata mpaghara nke ụbụrụ ụbụrụ ma weghachite ọrụ akwara, si otú a na-eme ka mmerụ ahụ strok dị mma.

Mmetụta immunomodulatory

Nnyocha achọpụtala na ndị MSC nwekwara ọrụ nchịkwa abụọ na nzaghachi mgbochi nke etiti mgbe ọrịa strok gasịrị: N'otu aka ahụ, ha nwere ike ịbawanye ọnụ ọgụgụ nke mkpụrụ ndụ B, mkpụrụ ndụ NK, na mkpụrụ ndụ T na ụbụrụ ụbụrụ, na-enwe mmetụta na-akwalite mgbochi. N'aka nke ọzọ, ọnụ ọgụgụ dị elu nke ihe ndị na-egbuke egbuke nwere ike ime ka ọrụ immunosuppressive nke MSC rụọ ọrụ, si otú ahụ na-eme ka mmetụta abụọ na-egbochi lymphocyte na-emepụta ihe na-emepụta ihe na-emepụta ihe na-emepụta ihe na-emepụta ihe na-emepụta ihe na-eme ka ọ bụrụ ihe na-adịghị mma.

Usoro nke apoptosis cell

Apoptosis cell bụ usoro dị mkpa nke mmerụ ahụ na-emerụ ahụ na ọrịa strok ischemic, na MSC nwere ike igbochi apoptosis nke neuronal, na-eme mmetụta na-adịghị mma. Usoro ọgwụgwọ MSC nwekwara ike ibelata ischemic ọrịa strok na-akpata astrocyte necrosis, na Ọzọkwa, hypoxia-preconditioned MSC na-egosipụta mmetụta ọgwụgwọ ka mma na mbenata necrosis sel nke ọbara ọgbụgba na-akpata.

Nyocha ụlọ ọgwụ nke ọgwụgwọ stem cell maka ọrịa strok ischemic.

Mgbanwe nke mkpụrụ ndụ sel mesenchymal (MSC) maka ọgwụgwọ ọrịa strok ischemic egosila nsonaazụ na-ekwe nkwa na ọmụmụ ihe izizi. Ndị nchọpụta mere nyocha meta-analysis nke ule ụlọ ọgwụ 20 gụnyere ngụkọta nke ndị ọrịa 1127, gụnyere 573 na otu nnwale (nke a na-emeso ya na MSCs) na 554 na otu njikwa (ọgwụgwọ ọdịnala). Tụnyere ọgwụgwọ a na-ahụkarị, ntụgharị MSC belatara nke ukwuu National Institutes of Health Stroke Scale (NIHSS) na 1, 3, ọnwa 6, na otu afọ mgbe ọgwụgwọ gasịrị. Ọ mekwara nke ọma ọrụ nke ndeksi ibi ndụ kwa ụbọchị na ọnwa 1 na 6, yana ọrụ moto na akara nnwere onwe arụ ọrụ na ọnwa 1, 3 na 6.

Isi ihe nleba anya gụnyere: (1) National Institutes of Health Stroke Scale (NIHSS) akara; (2) Ndekọ Barthel (BI); (3) Fugl-Meyer Assessment (FMA) akara; (4) Akara Independence Measure (FIM); na (5) Akara Rankin Scale (mRS) agbanweela.

Tụnyere ọgwụgwọ a na-ahụkarị, ọgwụgwọ ọrịa strok na-ebelata nke ukwuu NIH Stroke Scale (NIHSS) na ọnwa 3.

E jiri ya tụnyere ọgwụgwọ a na-ahụkarị, ọ nweghị nnukwu ọdịiche dị na akara Barthel Index (BI) na ọnwa 3 na ọgwụgwọ mesenchymal stem cell (MSC) maka ọrịa strok.

Tụnyere ọgwụgwọ a na-emekarị, ọgwụgwọ ọrịa strok mesenchymal stem cell (MSC) mere ka akara ngosi Functional Movement Assessment (FMA) dịkwuo mma na ọnwa 3.

Tụnyere ọgwụgwọ a na-emekarị, ọgwụgwọ ọrịa strok mesenchymal stem cell (MSC) mere ka akara ngosi Independence Measure (FIM) dị mma na ọnwa 3.

Tụnyere ọgwụgwọ a na-emekarị, ọgwụgwọ mesenchymal stem cell (MSC) maka ọrịa strok egosighi nnukwu ihe dị iche na akara Modified Rankin Scale (mRS) na ọnwa 3.

N'ịchịkọta ọnụ, nnukwu ọmụmụ ihe ọmụmụ ihe na-egosi na ọ ga-ekwe omume na ịdị irè nke ọgwụgwọ transplantation nke mesenchymal stem cell (MSC) na ọgwụgwọ ọrịa strok nke ischemic, nke ruo n'ókè ụfọdụ na-eme ka nhụjuanya akwara, ọrụ moto, na ọrụ nke ndụ kwa ụbọchị na ndị ọrịa nwere ischemic. ọrịa strok.

Ọdịnihu na atụmanya

N'ime afọ ndị na-adịbeghị anya, site na nghọta dị mfe na mgbanwe mgbanwe nke nchọpụta cell cell na-aga n'ihu na-emewanyewanyewanye na mgbasawanye nke usoro dị iche iche nke nchọpụta MSC, mmepe nke ubi nyocha mkpụrụ ndụ etolitela tozuru okè na ihe ọhụrụ. Ka ọ dị ugbu a, ọ bụghị naanị na nnwale anụmanụ gosipụtara mmetụta ọgwụgwọ nke mkpụrụ ndụ stem, kamakwa enwere ike ịme ọgwụgwọ MSC maka ọrịa strok ischemic. N'ọtụtụ ule ụlọ ọgwụ, ọgwụgwọ MSC maka ọrịa strok ischemic na-aga n'ihu.

Ka ọ dị n'oge na-adịbeghị anya, enweela ule ụlọ ọgwụ 101 debara aha na nchekwa data ụlọ ọgwụ US maka ọgwụgwọ stem cell maka ọrịa strok, yana ọmụmụ 25 metụtara ọgwụgwọ MSC maka ọrịa strok ischemic. Nnwale ụlọ ọgwụ egosila na MSC nwere ọganihu dị ukwuu na ọrụ akwara ozi maka ndị ọrịa strok ischemic, na-akwado ịdị irè ha na nchekwa ogologo oge.

N'ọdịnihu, a ga-enwekwu ọganihu ahụike gụnyere MSC nke a ga-atụgharị n'ime ngwa ụlọ ọgwụ, na-enye aka na ụlọ ọrụ ahụike na ichekwa ahụike na ọdịmma nke ndị mmadụ n'ụwa nile.