Nyeredzi mumaseru masero! Kutsvakurudza kwekiriniki uye kushandiswa kwembilical cord mesenchymal stem masero

Mumakore achangopfuura, tsvagiridzo yemesenchymal stem masero yanga ichiramba ichiwedzera, uye kusvika parizvino, zvinopfuura zvinyorwa zve47000 zvine chekuita nemesenchymal stem masero zvakatorwa paPubmed.

Umbilical cord mesenchymal stem masero agara achionekwa sepfuma nemasayendisiti uye ari kugara achiratidza kugona kwavo kurapwa kwechirwere uye kurwisa kuchembera.

Nhasi, mupepeti achakutsanangurira kuti umbilical cord mesenchymal stem cells chii, uye oratidza runako rweruvhuvhu mesenchymal stem masero mundima yezvokurapa nekusuma makiriniki ekushandiswa kwetsvakiridzo dzemaseru eruvhuvhu mesenchymal stem cells.

Chii chinonzi umbilical cord mesenchymal stem masero?

Umbilical cord mesenchymal stem cells (hUC MSCs) anobva paguvhu revana vachangozvarwa uye akachengetedzeka uye ari echinyakare kana achienzaniswa neakura mesenchymal stem cells. Vane kuwedzera kwakasimba, kusiyanisa, uye masimba ekudzivirira zvirwere; Pamusoro pezvo, zvichienzaniswa nezvimwe zvinopa mesenchymal stem masero, umbilical cord mesenchymal stem masero anowanikwa kubva akavharirwa tambo yeguvhu pasina kukuvadza kumuviri wemunhu. Naizvozvo, umbilical cord MSCs dzakakodzera kukiriniki yekutsvagisa uye kushandisa, uye ndiyo sarudzo inosarudzika yesero kurapwa uye mushonga wekuvandudza.

Umbilical cord mesenchymal stem masero akaratidza kukwanisa kusiyanisa zvakanyanya mu vitro, kusanganisira chondrocytes, adipocytes, osteoblasts, skeletal cells, cardiomyocytes, masero echiropa, pamwe nekubudisa masero eglucagon, somatostatin secreting cells, glial cells (oligodendrocytes), uye dopaminergic neurons. Naizvozvo, umbilical cord mesenchymal stem masero akanyatsokodzera kushandiswa kwekiriniki uye kutsvagisa kwesainzi.

Mesenchymal stem masero ave nzvimbo yekutsvagisa

Mumakore achangopfuura, tsvakiridzo yemesenchymal stem masero yanga ichikwira, uye ClinicalTrials kubva kuNIH muUnited States ndiyo yakakura pasi rose kiriniki yekutsvagisa dhatabhesi. Zvinoenderana nemhedzisiro yekutsvaga yedatabase, kubva muna Chivabvu 13, 2019, pakanga paine mazana manomwe nemakumi mashanu nenhatu zvidzidzo zvekiriniki zvakanyoreswa mudhatabhesi zvine chekuita nemesenchymal stem masero. Pakati pavo, nhamba yezvidzidzo zvekiriniki yakaitwa muChina, Europe, uye United States iri pakati pezvitatu zvepamusoro pasi rose.

Kubva pamaonero emhando dzezvirwere zvinorapwa, tsvakiridzo yekiriniki yemesenchymal stem cell inosanganisira mazana ezvirwere. Pakati pazvo, chirwere chetsinga, chemwoyo, uye chemapfupa ndidzo nhatu huru dzekutsvagisa nzvimbo, dzinoverengera kupfuura 15%, uye iyo yakazara inodarika hafu. Pamusoro pezvo, chikamu chechirwere cheshuga, chiropa, mapapu, gastrointestinal turakiti, ganda, autoimmune zvirwere uye graft versus host disease (GVHD) ingangoita 5%, inova yakakosha kukiriniki yekutsvagisa nzira ye mesenchymal stem masero.

Clinical application research case yeumbilical cord mesenchymal stem cells

01.Metabolic zvirwere

1.1 Chirwere cheshuga

Parizvino, hapana mushonga unorapa chirwere cheshuga mukiriniki, uye tinogona kungovimba ne insulin kana zvinodhaka in vitro kuderedza shuga yeropa, uye panguva imwe chete kushandira pamwe nekudya uye kurovedza muviri. Mesenchymal stem masero ane simba rekukurudzira pancreatic islets β Basa rekuvandudzwa kwesero.

MuChina, kune zvipatara zvitatu zvepamusoro zvakaita tsvakiridzo yekiriniki nezve mesenchymal stem cell transplantation mukurapa kwechirwere cheshuga chechipiri. Varwere vazhinji vakawana mhedzisiro yekudzikisa shuga yeropa uye insulin, uye vamwe vanotomira kutora mishonga. Panguva imwecheteyo, mamiriro emuviri wemurwere akavandudzwa uye mamiriro avo ehutano akagadziriswa kune imwe nzvimbo.

Fuzhou General Hospital yeNanjing Military Region yakaongorora kuchengetedzeka uye kushanda zvakanaka kweruvhuvhu mesenchymal stem masero akasanganiswa neautologous bone marrow mesenchymal stem masero mukurapa chirwere cheshuga cherudzi rwekutanga pasina immunotherapy. Mhedzisiro yacho yakaratidza kuti kurapwa kwevarwere makumi mana nevaviri kwaive kwakanaka. C-Pep yakawedzera ne105.7%, insulini 49.3%, HbA1c yakaderera ne12.6%, kukurumidza kweglucose yeropa yakaderera ne24.4%, uye insulin inoda yakaderera ne29.2%.

1.2 Matambudziko echirwere cheshuga

Mesenchymal stem masero ane tarisiro yakakura yekushandisa mune matambudziko echirwere cheshuga.

1.2.1 Chirwere cheshuga

Pachipatara cheShaanxi Provincial People's Hospital, pasi pemamiriro ezvinhu asina hutachiona, guvhu remesenchymal stem cell suspension rakaiswa mumhasuru panguva dze 3cm x 3cm pamaronda evarwere ve5 vane chirwere cheshuga chechipiri chakaomeswa nerutsoka rweshuga. Kune avo vane maronda akanyanya, majekiseni akapatsanurwa 1cm x 1cm mutsoka, achitariswa jekiseni rakatenderedza ronda. Mushure mekurapa, chiyero chemarwadzo etsoka uye chiveve chakaderedzwa zvakanyanya, kupindirana kwepakati kwakavandudzwa zvakanyanya, uye maronda akapora zvachose.

PaAffiliated Anhui Provincial Hospital yeAnhui Medical University, umbilical cord mesenchymal stem masero akaiswa munzvimbo dzemaronda emakumbo akabatwa evarwere makumi mashanu nenhatu vane giredhi II-IV vane chirwere cheshuga. Mushure memwedzi mitatu yekurapa, kana ichienzaniswa neboka rinodzora, boka rekurapa rakaratidza kuvandudzwa kukuru kwekushisa kweganda, ankle-brachial pressure index, ganda reokisijeni tension, uye kufamba kure.

1.2.2 Chirwere cheshuga nephropathy

The Clinical Medical College yeFuzhou General Hospital, Chechipiri Military Medical University, uye Affiliated Changzheng Hospital yeChipiri Military Medical University vakarapa varwere gumi nevashanu vane guvhu remesenchymal stem masero kuburikidza nekupindira mupancreatic dorsal artery, bilateral renal arteries, uye peripheral tsinga infusion. . Mushure mekurapwa, varwere vakaratidza kuvandudzwa kweBP, shuga yeropa, C-peptide mazinga, uye renal basa zviratidzo. Panyaya yekubudirira kwekiriniki mukuvandudza diastolic yeropa uye kushanda kwetsvo, boka rekurapa rakapfuura boka rekutonga raibatwa nemuromo telmisartan pamwe ne subcutaneous insulin jekiseni.

02.Neurological disorders

2.1 Chirwere cheParkinson

Mhosva yekutanga yekuisirwa stem cell yechirwere cheParkinson muChina yakapedzwa muchipatara cheShijiazhuang. Xie Xiyun, ane makore makumi manomwe nematanhatu okukura anobva kuIndonesia, anga achirwara nechirwere cheParkinson kweanopfuura makore gumi, achinetseka nekuomarara zvigunwe zvitatu zveruoko rwake rwerudyi, kusimba kwemuviri, kuoma kwekumedza, uye kurasikirwa nekugona kuzvitarisira. Mumaawa mashoma ekugamuchira mesenchymal stem cell transplantation, akakwanisa kudya zvakanaka uye zvakanaka achiona terevhizheni manheru iwayo, nekuvandudzwa kwakanyanya mune zvimwe zviratidzo.

Dr. Sun Li vanobva kuChina Medical University vakaisa kumiswa kune umbilical cord mesenchymal stem masero mutsinga dzemutsipa dzevarwere gumi vechirwere cheParkinson. Mushure mekusimwa, varwere vakaratidza kuvandudzwa kwekufamba kwekufamba, kudengenyeka kunotyisa, kufamba kwekuzvidira, uye kukwanisa kugara nekumira. Hazvina kungoita zvinobudirira kudzora kufambira mberi kwehosha yeParkinson, asi zvakare yakavandudza zvakanyanya basa reakakuvadzwa uropi tishu, nokudaro kuwedzera hupenyu hwevarwere.

2.2 senile dementia

Chikwata chinotungamirwa naZhou Qiang paJinyang Hospital muGuiyang City vakarapa varwere vana vakura vane dementia vachishandisa autologous bone marrow stem cells. Murwere wekutanga, murume ane makore makumi manomwe neshanu, haana kuziva nhengo dzemhuri pakubvumidzwa uye akarasikirwa nedundira uye kudzora ura. Pashure pokurapwa kwaakanga aitwa, zviratidzo izvi zvakakurumidza kuva nani, uye pakubuda, haana kungoziva nhengo dzemhuri yake chete asiwo akatangazve kushanda zvakanaka kwedundira nouura.

2.3 Kushaya hope

Dr. Wang Yali vanobva kuZhejiang Armed Police Hospital vakarapa varwere ve19 vane kushaya hope kuburikidza nekupinda mutsinga yeumbilical cord mesenchymal stem cells. Varwere ava vese vakasangana nedambudziko rekurara, kuderera kwekurara, uye nguva yekurara yakazara isingasviki maawa matanhatu. Mushure memwedzi mumwe wekurapa, kuvandudzwa kwehutano hwekurara muvarwere kwaive kwakafanana neyomushonga wemuromo. Mushure memwedzi miviri yekurapwa, zvibodzwa zvekurara zvevarwere zvaive nani pane zvemushonga wemukanwa. Mushure memwedzi mitatu yekurapwa, hutano hwevarwere hwakanga huri nani zvikuru pane hwemushonga wemukanwa, uye migumisiro yekurapa kumwe chete inogona kusvika kune rimwe gore, zvakanyanya kuvandudza kurara kwevarwere.

03.Musculoskeletal disorders

3.1 Degenerative joint disease

Mumakore ekuma1990, Brittberg nevamwe vakatanga kuwana mimwe mibairo mukugadzirisa kukuvara kwecartilage vachishandisa autologous chondrocytes. Naizvozvo, zvidzidzo zvakawanda zvakatanga kutarisa mesenchymal stem masero. Mesenchymal stem masero anogona kusiyanisa kuita akasiyana matishu uye kugadzirisa articular pamusoro cartilage uye subchondral bone kukuvara.

Muchidzidzo chakaitwa naAkgun et al. kubva kuDhipatimendi reOrthopedics uye Traumatology paYunivhesiti yeIstanbul muTurkey, vakaenzanisa maitiro ekurapa e autologous bone marrow mesenchymal stem cells uye chondrocytes muvarwere ve14 vane osteoarthritis. Vakaona kuti varwere vanogamuchira mesenchymal stem cell kurapwa vakaratidza kuvandudzwa kwakakosha mukukuvara kwemabvi uye osteoarthritis mhedzisiro scores (KOOS) uye kuona analog scale (VAS) scores.

Wakitani et al. kubva kuFeinberg School of Medicine kuNorthwestern University uye Cleveland Clinic Hospital paOhio University vakaita ongororo yekiriniki vachishandisa mesenchymal stem masero. Muboka rekuedza, collagen gel yakavhenganiswa ne autologous bone marrow-yakagadzirwa mesenchymal stem masero akaiswa mukati mevarwere' articular cartilage maronda, nepo boka rekutonga rakawana chete yakaenzana yakaenzana ye collagen gel. Mapoka maviri aya akaratidza kuvandudzwa kwebasa rekubatana, asi boka rekuedza chete rakaratidza kuvandudzwa kwe cartilage yakajeka panzvimbo yekukuvara. Vakatevera nevarwere makumi mana nerimwe vakagamuchira mesenchymal stem cell therapy kweanosvika makore gumi nerimwe uye havana kuwana matambudziko akakomba.

04.Zvirwere zvekuzvara

4.1 Kushaya mbereko

Kugadzirisa kukuvadzwa kwakanyanya kune endometrium, inova dambudziko revakadzi vazhinji vasina mbereko, ikozvino zvinogoneka kuburikidza ne mesenchymal stem cell regenerative tekinoroji, zvichiunza tariro yekuzadzisa zviroto zvevakadzi zvekuva amai.

Chikwata chaProfessor Hu Yali kubva kuDhipatimendi reObstetrics uye Gynecology yeGulou Hospital muNanjing, vachibatana neboka raProfessor Dai Jianwu kubva kuInstitute of Genetics and Developmental Biology yeChinese Academy of Sciences, vakabudirira kugadzirwa, kekutanga pasi rose, nzira yekubatanidza. collagen scaffolds ine mesenchymal stem masero. Nekubatanidza echinyakare hysteroscopic matekiniki, vakawana mashandiro ekugadzirisa endometrium yakakuvadzwa, vachibatsira varwere vatatu kuzadzisa zviroto zvavo zvekuve vanaamai.

Ms. Hu vanobva kuYancheng, Jiangsu, vakawana miscarriges matatu echisikigo pakati pemakore 31 ne34, zvichiita kuti pave nekunamatira kwakanyanya muuterine cavity nekuda kwekuvhiyiwa kwepashure kwepamuviri uterine curettage. Ichishandisa mesenchymal stem masero, Gulou Hospital muNanjing yakabudirira kugadzirisa endometrium yake mukati memwedzi misere. Izvi zvakaita kuti akwanise kubata pamuviri zvinobudirira, uye musi waChikunguru 17, 2014, akabereka mwana wekutanga we "regenerative mushonga mwana" muChina.

Chipatara cheNanjing Gulou's mesenchymal stem cell "endometrial regeneration surgery" chakabudirira kugonesa varwere gumi nevatatu vasina mbereko vane uterine cavity yakakomba inonamira kubata pamuviri uye kuzvara, zvichiita kuti pave nehwerengedzo yegumi nevana vane hutano "regenerative mushonga vacheche."

4.2 Kukundikana kweovarian nguva isati yakwana

Premature ovarian failure inoreva chiitiko icho vakadzi vanoona ovarian atrophy uye vanomira ovulating vasati vasvika makore makumi mana. Parizvino, inenge 1% yevakadzi vezera rekubereka munyika yose vanoona kukundikana kwe ovarian nguva isati yakwana, zvichiita kuti ive chikonzero chikuru chekushaya mbereko kwevakadzi.

Muzvinafundo Wu Ji nechikwata chavo vanobva kuShanghai Jiao Tong University kuChina vakatsaura masero echikadzi ekuberekesa kubva pavhiki imwe kusvika kune maviri-ekare egirinhi fluorescent protein transgenic mbeva. Mushure mekucheneswa, tsika, uye mamwe maitiro, maseru aya akaisirwa mumakonzo aine nguva isati yakwana ovarian kutadza. Mhedzisiro yekutsvagisa yakaratidza kuti masero ekubereka echikadzi, semamwe masero, ane zvimiro zvemba. Mushure mekugara, mamwe masero akasiyaniswa uye zvishoma nezvishoma akakura kuita maoocyte akakura, nokudaro achidzoreredza ovarian basa.

4.3 Erectile dysfunction (ED)

Kushandisa stem cell therapy ye erectile dysfunction inogona kutsiva yakakuvara kana yakafa penile tissue masero uye kuvanza zvinhu zvekugadzirisa kushanda kwepenile tissue masero.

Vane gumi neshanu erectile dysfunction varwere muDenmark vakagamuchira mesenchymal stem cell infusions pasina mishonga kana penile transplantation. Pashure pemwedzi mitanhatu, varume vasere vakakwanisa kupinda muvatano senguva dzose.

05.Autoimmune zvirwere

Muna 2007, Muzvinafundo Sun Lingyun kuChina akabudirira kubata nyaya mbiri dzerefractory systemic lupus erythematosus vachishandisa mesenchymal stem masero, uye gore rakatevera, akarapa dzimwe pfumbamwe. Mushure mekurapa, zviratidzo zvevarwere zvakaderedzwa zvakanyanya, uye pakanga pasina kudzoka zvakare panguva yekutevera kwemwedzi we6.

Muna 2012, Dhipatimendi reRheumatology neImmunology reJiangsu University Affiliated Hospital rakapinza mutsinga wembilical cord mesenchymal stem cells kune varwere makumi maviri nemumwe vane systemic lupus erythematosus, izvo zvakaderedza zvakanyanya kuwanda kweprotein yeurinary muvarwere ava, kuderera kwechirwere, uye kudzikisa Systemic Lupus Erythematosus. Chirwere Chiitiko Index.

Mhedzisiro yemesenchymal stem cell transplantation pachiitiko chechirwere uye urinary protein mune varwere vane systemic lupus erythematosus (SLE)

Muna 2013, Dhipatimendi reRheumatology uye Immunology yeFirst Affiliated Hospital yeYangtze University neJingzhou First People's Hospital yakapa umbilical cord mesenchymal stem masero kuburikidza neanterior cubital vein kuvarwere gumi nevasere vane systemic lupus erythematosus. Mwedzi mumwe gare gare, varwere vazhinji vakaratidza kuvandudzwa kukuru kwezviratidzo zvekiriniki uye zviratidzo zvakadai sefivha, kuneta, uye kushaya simba zvichienzaniswa nepamberi pekusimwa.

06.anti-kuchembera

Kuchembera kwemuviri wemunhu kune hukama nekusakwana kana kusashanda stem cell huwandu. Sezvo zera richiwedzera, kune kuderera kwemaitiro muhuwandu uye kunaka kwemasero emasero mumuviri. Maitiro aya anotungamira mukudzikira uye kuchembera kwemaseru akakuvadzwa uye nhengo uye matishu anoumbwa nemasero, zvichikonzera hutano husina kunaka uye kuchembera. Mesenchymal stem masero ane mukana wekusiyanisa akawanda, anokwanisa kusiyanisa mumhando dzakasiyana dzemaseru anodiwa mumuviri. Nekuwedzera masero asina kukwana ari mumuviri uye kugadzirisa nhengo dzakakanganisika uye matishu, mesenchymal stem maseru anoita basa mukurwisa kuchembera.

Nekufarira kuri kuwedzera uye kushandiswa kweumbilical cord mesenchymal stem cell research, zvinotendwa kuti munguva pfupi iri kutevera, ichashandiswa zvakanyanya mumakiriniki, zvichipa kukosha kukuru kune hutano hwevanhu.